Amit Joshi, Wolfgang Bangerth, Ruchi Sharma, John Rasmussen, Wei Wang, and Eva
Sevick
Molecular tomographic imaging of lymph nodes with NIR
fluorescence
Proceedings of the IEEE International
Symposium on Biomedical Imaging, Arlington, VA, 2007, pp. 564-567.
This contribution describes a system and method for tomographically imaging
lymph nodes marked with a lymph endothelium targeting fluorescence reporter.
A novel scanning NIR laser line source was employed to acquire multiple sets
of boundary fluorescence measurements in the groin region of a Yorkshire
swine. The source light shaped into a linear profile was modulated at 100MHz
and a gain modulated homodyne ICCD detection system was used to collect area
measurements of fluorescence amplitude and phase on the illumination
plane. Multiple measurement data sets generated by scanning the excitation
sources were processed simultaneously to generate the interior fluorescence
distribution in tissue by implementing a parallelized dual adaptive finite
element based fluorescence tomography algorithm. Three dimensional
localization of the approximately 2.5cm deep inguinal lymph node in swine
groin following injection of $100\mu{L}$ of lymph targeting
Hyaluronan-Indocyanine Green conjugate dye is demonstrated.
Wolfgang Bangerth
Sat Apr 20 09:13:53 MDT 2024