Kildong Hwang, Tianshu Pan, Amit Joshi,
John C. Rasmussen, Wolfgang Bangerth, Eva M. Sevick-Muraca
Influence of excitation light rejection on
forward model mismatch in optical
tomography
Physics in Medicine and Biology, vol. 51 (2006), pp. 5889-5902.
Fluorescence enhanced tomography for molecular imaging requires low
background for detection and accurate image reconstruction. In this
contribution, we show that excitation light leakage is responsible for
elevated background and can be minimized with the use of gradient
index (GRIN) lenses when using fibre optics to collect propagated
fluorescence light from tissue or other biological media. We show that
the model mismatch between frequency-domain photon migration (FDPM)
measurements and the diffusion approximation prediction is decreased
when GRIN lenses are placed prior to the interference filters to
provide efficient excitation light rejection. Furthermore, model
mismatch is correlated to the degree of excitation light leakage. This
work demonstrates the importance of proper light filtering when
designing fluorescence optical imaging and tomography.
Wolfgang Bangerth
Sun Mar 3 06:42:12 MST 2024