Amit Joshi, Wolfgang Bangerth, Eva M. Sevick-Muraca
Non-contact fluorescence optical tomography
with scanning patterned illumination
Optics Express, vol. 14 (2006), pp. 6516-6534.
This article describes a novel non-contact fluorescence optical tomography
scheme which utilizes multiple area illumination patterns, to reduce the
ill-posedness of the inverse problem involved in recovering interior
fluorescence yield distributions in biological tissue from boundary
fluorescence measurements. The image reconstruction is posed as an
optimization problem which seeks a tissue optical property distribution
minimizing, for all illumination patterns simultaneously, a regularized
difference between the observed boundary measurements of
light distribution, and the boundary measurements predicted from a physical
model. Multiple excitation source illumination patterns are described by
line and Gaussian sources scanning the simulated tissue phantom surface and
by employing diffractive optics-generated patterns. Multiple measurement data sets generated by scanning excitation sources are processed simultaneously to generate the interior fluorescence distribution
in tissue by implementing the fluorescence tomography algorithm in a
parallel framework suitable for multiprocessor computers. Image
reconstructions for single and multiple fluorescent targets (5mm diameter)
embedded in a 512ml simulated tissue phantom are demonstrated, with depths
of the fluorescent targets from the illumination plane between 1cm
to 2cm. We show both qualitative and quantitative improvements of our
algorithm over reconstructions from only a single measurement.
Wolfgang Bangerth
Sun Mar 3 06:42:12 MST 2024