Amit Joshi, Wolfgang Bangerth,
Kildong Hwang, John C. Rasmussen, Eva M. Sevick-Muraca
Fully adaptive FEM based fluorescence optical
tomography from time-dependent measurements with area
illumination and detection
Medical Physics, vol. 33 (2006), pp. 1299-1310.
Using an area-illumination and area-detection
scheme, we acquire fluorescence frequency domain measurements from a tissue
phantom with an embedded fluorescent target, and obtain
tomographic reconstructions of the interior fluorescence absorption map
with an adaptive finite element based scheme. The tissue phantom
consisted of a clear acrylic cubic box (512ml) filled with 1% Liposyn
solution, while the fluorescent targets were 5mm diameter glass bulbs
filled with 1μM Indocyanine Green dye solution in 1%
Liposyn. Frequency domain area illumination and detection employed
a planar excitation source using an expanded intensity
modulated (100MHz) 785 nm diode laser light and a gain modulated image intensified
charge coupled device camera, respectively. The excitation pattern was
characterized by isolating the singly scattered component with cross
polarizers, and was input into a dual adaptive finite element-based scheme for
three dimensional reconstructions of fluorescent targets embedded beneath the
phantom surface. Adaptive mesh refinement techniques allowed efficient
simulation of the incident excitation light and the reconstruction of
fluorescent targets buried at the depths of 1cm and 2cm. The results
demonstrate the first clinically relevant non-contact
fluorescence tomography with adaptive finite element methods.
Wolfgang Bangerth
Sat Apr 20 09:13:53 MDT 2024